@conference {40947,
	title = {The measurement of ovine follicle stimulating hormone (FSH) bioactivity utilizing a recombinant human FSH receptor bioassay.},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {57},
	year = {1997},
	month = {Jan},
	pages = {243-245},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {Recently a bioassay for human FSH has been described based on FSH stimulation of cyclic AMP production by a Chinese hamster ovary (CHO/FSH-R) cell line stably expressing the human FSH receptor (FSH-R cells). In this study, the viability of this bioassay for measuring ovine FSH was examined. Freshly thawed FSH-R cells were bulked up in culture, and aliquots of 250,000 cells/well dispensed into 48 well culture dishes and incubated overnight at 37{\textdegree}C. The assay culture media was then replaced with 0.25 ml fresh media (α-MEM + 0.1\% BSA + 0.25 mM 3-isobutyl-1-methyl-xanthine) containing varying doses of FSH preparations, and the cells incubated for 4 h at 37{\textdegree}C. The assay culture media was then removed and stored frozen at -20{\textdegree}C until assayed for cyclic AMP by radio immunoassay.},
	issn = {1176-5283},
	author = {Young W and Henderson K and Hudson N and Moore L and Albanese C}
}
