@conference {40549,
	title = {Anthelmintic resistance in the New Zealand Animal Production Industries},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {54},
	year = {1994},
	month = {Jan},
	pages = {1-4},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {Enzymeimmunoassays (EIAs) for oestradiol-17b and progesterone were  developed using antibody-coated microtitre plates, and steroid-horseradish  peroxidase conjugates as steroid {\textquoteright}tracers{\textquoteright}. The characteristics of standard  curves and the precision of measurements were comparable between the EIAs  and corresponding radioimmunoassays (RIAs). The applicability of the EIAs  was demonstrated by using them to measure oestradiol-17b and progesterone  concentrations in media sampled daily from cultures of bovine granulosa cells.   The EIAs detected the characteristic switch in cellular steroidogenic capacity  from oestradiol-17b to progesterone production typical of cultured granulosa  cells. There was a significant linear correlation (P<0.001) between the EIAs  and RIAs in the concentrations of steroids measured in granulosa cell culture  medium by the two immunoassay formats. These findings may encourage  other laboratories to consider converting "in-house" RIAs to EIAs, thereby  eliminating the potential health and environmental hazards associated with the  radioisotopes required for RIA.  },
	issn = {1176-5283},
	author = {Watson TG}
}
