@conference {40562,
	title = {Measurement of steroids by enzymeimmunoassay: An environmentally friendly alternative to radioimmunoassay},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {54},
	year = {1994},
	month = {Jan},
	pages = {55-58},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {Enzymeimmunoassays (EIAs) for oestradiol-17b and progesterone were  
developed using antibody-coated microtitre plates, and steroid-horseradish  
peroxidase conjugates as steroid {\textquoteright}tracers{\textquoteright}.  The characteristics of standard  
curves and the precision of measurements were comparable between the EIAs  
and corresponding radioimmunoassays (RIAs).  The applicability of the EIAs  
was demonstrated by using them to measure oestradiol-17b and progesterone  
concentrations in media sampled daily from cultures of bovine granulosa cells.   
The EIAs detected the characteristic switch in cellular steroidogenic capacity  
from oestradiol-17b to progesterone production typical of cultured granulosa  
cells.  There was a significant linear correlation (P<0.001) between the EIAs  
and RIAs in the concentrations of steroids measured in granulosa cell culture  
medium by the two immunoassay formats.  These findings may encourage  
other laboratories to consider converting "in-house" RIAs to EIAs, thereby  
eliminating the potential health and environmental hazards associated with the  
radioisotopes required for RIA. },
	issn = {1176-5283},
	author = {Henderson KM and Hardie AHM and Camberis M}
}
