@conference {41841,
	title = {Zearalenone challenge in sheep : urine sampling to measure response},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {65},
	year = {2005},
	month = {Jan},
	pages = {316-319},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	address = {Christchurch},
	abstract = {Zearalenone is a fungal toxin produced by Fusarium species commonly found on autumn pastures in New Zealand. It may interfere with reproductive functions in sheep. In a search for a genetic measure of susceptibility, ewe and ram lambs were dosed orally with a suspension of zearalenone at a series of rates, at 8 and 11 months of age. Urine samples were taken at intervals from 2 to 50 hours later. The concentration of urinary zearalenone and its metabolites (Zen) was assayed using an ELISA. Adjustment for urinary volume was made by measuring the urinary concentration of creatinine (Cr), and results were expressed as Zen/Cr ratio (loge-transformed). In a separate experiment, breeding ewes were dosed with zearalenone at a single rate, and Zen/Cr response was measured. The following effects were evaluated in lambs: a) sex difference (ewe versus ram lambs), b) responses to three dose rates, c) time-series response data. The heritability of Zen/Cr was determined in lambs and breeding ewes; results were analysed after standardising for each treatment and sampling group. Urinary Zen/Cr, 6 h after dosing, differed between ewe and ram lambs by a factor of 1.75 (ewe lambs higher; P < 0.01). Zen/Cr values for three dose rates (3, 6 and 9 mg zearalenone per lamb), averaged over three sampling time points after dosing, were 23.5, 43.2 and 59.3, respectively (P < 0.0001). Zen/Cr values for three sampling time points (2, 4 and 6 h after dosing), averaged over three dose rates, were 58.0, 35.8 and 29.0, respectively (P < 0.0001). By 50 h after dosing, the Zen/Cr average had fallen to 4\% of its 2-h value. The interaction between dose rate and time of urinary sampling was not significant for log Zen/Cr. A preliminary estimate of the heritability for standardised Zen/Cr was 0.32 {\textpm} 0.10 (P < 0.01). The dosing regime reported here could be used for a test of animal response to zearalenone in ram breeders{\textquoteright} flocks.
KEYWORDS: sheep; zearalenone; dose response; urine; heritability. },
	issn = {1176-5283},
	author = {Morris CA and Amyes NC and Sprosen JM and Hawkes AD and Finch SC and Miles CO}
}
