@conference {40678,
	title = {Evaluation of {\textquoteright}CONFIRM{\textquoteright} as a method to verify pregnancy in dairy cows},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {55},
	year = {1995},
	month = {Jan},
	pages = {91-93},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {A total of 24 mature rams and ewes (>2.5 yrs) from a line selected for  high backfat thickness and an unselected control line were used to investigate  the relationship between maintenance efficiency and body composition in  mature sheep. Over a 6 week period the sheep were fed daily a pelleted  ration at levels to maintain liveweight. At the end of this period the weights  of total fat, carcass lean, viscera and of the empty body (fleece free) were  estimated from X-ray computed tomographic images. After adjustment for  empty body weight the control line required 5\% more feed to maintain  liveweight (P<0.05) than sheep from the fat line and males required 19\%  more feed (P0.05) than females. After adjustment for differences in either  total fat or carcass lean weight, there were no significant difference (P>0.05)  in feed requirements between the lines. This indicated that although a  genetic change in body composition did alter maintenance feed requirements  at maturity, the efficiency of maintaining a unit weight of either fat or carcass  lean remained the same. After adjustment for differences in total fat and  carcass lean weight, rams still required 11\% more feed than ewes to maintain  liveweight. Adjusting for visceral weight had no effect on feed requirements  to maintain the mature sheep. It was proposed that differences in the  turnover rates of adipose tissue or carcass lean contributes to the higher  maintenance feed cost observed for the mature ram.  },
	issn = {1176-5283},
	author = {Henderson KM and Camberis M and Macmillan KL}
}
@conference {40562,
	title = {Measurement of steroids by enzymeimmunoassay: An environmentally friendly alternative to radioimmunoassay},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {54},
	year = {1994},
	month = {Jan},
	pages = {55-58},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {Enzymeimmunoassays (EIAs) for oestradiol-17b and progesterone were  
developed using antibody-coated microtitre plates, and steroid-horseradish  
peroxidase conjugates as steroid {\textquoteright}tracers{\textquoteright}.  The characteristics of standard  
curves and the precision of measurements were comparable between the EIAs  
and corresponding radioimmunoassays (RIAs).  The applicability of the EIAs  
was demonstrated by using them to measure oestradiol-17b and progesterone  
concentrations in media sampled daily from cultures of bovine granulosa cells.   
The EIAs detected the characteristic switch in cellular steroidogenic capacity  
from oestradiol-17b to progesterone production typical of cultured granulosa  
cells.  There was a significant linear correlation (P<0.001) between the EIAs  
and RIAs in the concentrations of steroids measured in granulosa cell culture  
medium by the two immunoassay formats.  These findings may encourage  
other laboratories to consider converting "in-house" RIAs to EIAs, thereby  
eliminating the potential health and environmental hazards associated with the  
radioisotopes required for RIA. },
	issn = {1176-5283},
	author = {Henderson KM and Hardie AHM and Camberis M}
}
@conference {40506,
	title = {An enzymeimmunoassay to measure oestrone sulphate in cows{\textquoteright} milk},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {53},
	year = {1993},
	month = {Jan},
	pages = {271-274},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {Romney rams selected for high wool staple strength grew 25\% more wool than rams selected for low staple strength when fed near maintenance rations during a 10 week winter experiment. Wool growth responses to supplements of energy (6\% of diet as tallow), protein (9\% of diet as bloodmeal) and methionine (1.2 g/d by intraperitoneal injection) were similar in the two staple strength selection lines. Tallow increased the estimated supply of metabolic energy by 53\% but was without effect on wool growth.  Bloodmeal increased dietary protein by 82\%, dietary energy by 9\% and sulphur supplies by 15\% and produced 38\% more wool. Methionine injections also increased sulphur supplies by 13\% but had no effect on energy or protein supplies and the wool growth response (35\%) was similar to that of bloodmeal. The finding that methionine supplies limited wool growth is consistent with other studies in sheep on low planes of nutrition. A component of wool growth responses was an increase in minimum mean fibre diameter. It accounted for most differences in staple strength between treatments but comparison at the same minimum mean fibre diameter indicated that the methionine supplement increased staple strength through an additional mechanism. Staple strength responses to nutritional treatments were similar in the two staple strength selection lines. It is concluded that the greater wool growth of the high staple strength selection line accounted for the majority of its staple strength advantage over the low strength line, and that there may be an effect of methionine on wool strength that is additional to its effect on fibre diameter. Because nutritional and genetic effects on staple strength were independent, they can be exploited concurrently to reduce the incidence of tender wools.},
	issn = {1176-5283},
	author = {Henderson KM and Camberis M and Simmons MH and Starrs WJ}
}
