@conference {78977,
	title = {Milk somatic cells are not suitable biomarkers of lactating ruminant mammary gland function},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {72},
	year = {2012},
	month = {Jan},
	pages = {3-7},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	address = {Christchurch},
	abstract = {<p>Understanding the molecular regulation of milk production by the ruminant mammary gland may help farmers meet increasing production milestones. Investigating these mechanisms currently relies upon invasive sampling by post-mortem or biopsy. The objective of this study was to determine if milk somatic cells, shed by the gland during milking, could be used as a non-invasive source of cells to measure these mechanisms. To answer this we employed two widely used molecular techniques, biochemical indices and quantitative polymerase chain reaction (qPCR). Experiment 1: Biochemical indices as measures of cell size, protein production capacity and efficiency were determined in milk somatic cells and mammary tissue harvested from six lactating dairy goats. Results showed protein production capacity (P = 0.03) and cell size (P = 0.03) were higher in mammary tissue compared to somatic cells while protein production efficiency was unaffected (P = 0.55). Experiment 2: Milk protein and ribosomal RNA gene expression were measured using qPCR in milk somatic cells and mammary tissue collected from three lactating dairy cows treated with growth hormone and four treated with saline. Results showed expression of all genes differed between milk somatic cells and mammary tissue. Taken together, these results indicate milk somatic cells are not suitable for measuring biochemical indices or gene expression in the lactating ruminant mammary gland.</p>
},
	issn = {1176-5283},
	author = {Sciascia Q and Pacheco D and Senna Salerno M and Blair HT and McCoard SA}
}
@conference {79023,
	title = {Relationship between profiles of free amino acids in fetal and maternal plasma with those in skeletal muscle, in twin and single fetuses from ad-libitum fed ewes in late gestation},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {72},
	year = {2012},
	month = {Jan},
	pages = {213-215},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	address = {Christchurch},
	abstract = {Skeletal muscle growth depends on amino acid (AA) availability, not only as building components, but also as regulatory signals for protein accretion (Wu 2009). However, their signalling role for muscle growth in ruminants is poorly understood. We have previously reported that lower muscle weight in twin compared to single fetuses in late gestation, is associated with changes in the profile of specific intracellular muscle free AA (FAA), when ewes are fed a maintenance plane of nutrition during gestation (Pacheco et al. 2010a). This suggests that some AA may regulate muscle growth during fetal life.},
	issn = {1176-5283},
	author = {Sales FA and Treloar BP and Pacheco D and Blair HT and Kenyon PR and Nicholas G and Senna Salerno M}
}
@conference {69500,
	title = {Effects of restricted fetal nutrition in utero on mTOR signaling in ovine skeletal muscle},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {70},
	year = {2010},
	month = {Jun},
	pages = {180-182},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	address = {Palmerston North},
	abstract = {High prolificacy (twins and triplets) is a desirable trait in sheep under intensive management systems. However, multiple-born lambs have lower birth weights resulting from placental insufficiency (McCoard, 1997), and carryover detrimental effects on lean deposition, feed efficiency, and lifelong performance compared to singletons (Greenwood et al., 1998; De Blasio  et al.,2007), suggesting differential nutritional programming in utero.},
	issn = {1176-5283},
	author = {Sciascia Q and Pacheco D and Bracegirdle J and Berry C and Kenyon PR and Blair HT and Senna Salerno M and Nicholas G and McCoard SA}
}
