Abstract

DNA samples from 691 mixed-sex Perendale sheep, born between 1998 and 2001, were typed for variation in the KRT33A gene using polymerasechain reaction - single-strand conformational polymorphism. The sheep were the progeny of two linesselected for either decreased (L), or increased (H) core bulk at yearling shearing, crosses between the lines and back-crosses. Five variants (A–E) of KRT33Awere identified with a frequency of 0.07, 0.04, 0.07, 0.35 and 0.47, respectively. The proportion of Adecreased and Bincreased, with a progressive increase incrimp frequency, fibre curvature and core bulk and a progressive decrease infleece weight, staple length and washing yield with each step-wise increase in the proportion of genes derived fromthe H line. An effect of the presence of Aon the measured wool characteristics was found, but was less than the effect with direct selection for core bulk. There was no consistent effect on body growth. While none of the variants of KRT33Awere strongly predictive of variation in core bulk, other genes on Chromosome 11 may be implicated in the differential expression of characteristics associated withcore bulk.

RMW, Sumner, RHJ Forrest, H Zhou, HV Henderson, and JGH Hickford

Proceedings of the New Zealand Society of Animal Production, Volume 73, Hamilton, 158-164, 2013