An enzyme-linked immunosorbent assay (ELISA) system has been developed for the detection of an endophytic fungus of Lolium perenne. The technique has been used to demonstrate quantitative differences in the spatial and tissue distribution of the endophyte in vegetative and reproductive tillers of infected plants. The influence of this on pasture toxicity is discussed. The nature of the endophyte antigen is described and the relationship between the antigen and toxicity discussed.
Proceedings of the New Zealand Society of Animal Production, Volume 44, , 177-180, 1984
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