Diseases affecting mucosal surfaces, especially gastrointestinal disorders, cause high mortality rates in young domestic livestock. Hyperimmune milk products, using milk secretions from ruminants immunised systemically with enteric pathogens, have been shown to confer passive immunity against such disorders but the results are variable. To study the type and titre of immunoglobulin (Ig) classes produced in milk secretions after immunisation with gut pathogens, we have developed ELISAs for the estimation of ovine IgG and IgA antibodies directed against E. coli. Utilising an indirect ELISAs technique, the method involved coating antigen onto microtitre plates then sequential addition of test sample dilutions (containing primary antibody), anti-class-specific antibody and anti- species horse radish peroxidase labelled antibody. Colorimetric end point detection employed 3, 3
Proceedings of the New Zealand Society of Animal Production, Volume 55, , 176-178, 1995
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